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CUSTOMER SPOTLIGHT
LabCentral: BioTek Supports A Better Way for Life Sciences Startups
Featuring: ELx405
Lab CentralDr. Johannes Fruehauf is dedicated to helping life science and biotech startup companies transform novel scientific concepts into therapeutic products. When founding his first pharmaceutical venture several years ago, he remembers the huge cost and time investments needed to find space and build a laboratory. He says, "It wasn't a good use of our resources. We would have realized greater value if the money and time had gone into our actual research efforts." He knew that other life science and biotech startups were facing the same frustrations and obstacles, and sought ways to improve the startup process. Years later, Dr. Fruehauf founded Cambridge Biolabs as a contract research organization in Cambridge, MA, and most recently co-founded the non-profit incubator, LabCentral, also in Cambridge, along with Peter Parker and Tim Rowe.

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FEATURED APPLICATION

Automated Hemocytometer-Based Live/Dead Cell Counting using Phase Contrast and Color Brightfield Imaging

Featuring: Cytation 5

Cell counting is one of the most common laboratory tasks in the life sciences. It is required for routine cell culture propagation and as a starting point for any cell-based assay. While there are many instruments available for automated cell counting, the reusable hemocytometer remains the inexpensive tool of choice. It is used with another common tool of the laboratory, a brightfield optical microscope such that cells can be visualized and counted on a grid of squares.

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TEK CORNER

Automated Color Imaging of Tissues

Featuring: Cytation 5

The microscopic examination of samples and tissues is one of the most commonly used tools to investigate cellular structure of plant and animal tissues and is the hallmark of clinical diagnostic pathology. The tissues are typically stained with compounds to improve contrast resulting in colored specimens that can be optically examined. Here we describe the use of the Cytation 5 Cell Imaging Reader to perform color brightfield imaging on fixed and stained tissues on microscope slides.

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PRODUCT SPOTLIGHT

MultiFlo FX Multi-Mode Dispenser

MultiFlo FXMultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates, with BioTek's unique Parallel Dispense™ technology to dispense up to four independent reagents in parallel without potential carryover. MultiFlo FX becomes a versatile multi-mode dispenser with the addition of either the RAD™ technology for random access dispensing to 6- to 384-well plates or a wash module for 6- to 384-well plate washing. Fast, intuitive programming and operation are via the color touch screen user interface. A MultiFlo FX configured with either RAD technology or the wash module replaces up to five liquid handlers, saving bench space and the budget.

Save even more with our "Make the Switch" special offer! Trade in a Multidrop or WellMate for up to $5,000 towards a MultiFlo FX. Promo valid until April 3, 2015.

TEK TIPS

arrow BioTek's Gen5 software controls all of our current microplate readers, several of which support Plug & Play connection.

In Gen5 | Instrument Configuration, the user can select COM port or Plug & Play connection. If you experience communications problems with the reader and Gen5 when connected via the COM port, try disconnecting and re-connecting using Plug & Play.

arrow When upgrading Gen5 for use with a Cytation instrument, it is important to say "yes" to the "upgrade existing databases" prompt.

This will ensure that new Optics Library files are imported so that you may be able to use new accessories such as objectives, LED and imaging cubes and adapters. Also, new imaging cube definitions may be available, such as different shades of red to differentiate between multiple red fluorophores.

arrow The barcode scanner (part number 7310017) can be installed onto a BioStack for use with BioTek microplate readers (under Gen5 computer control), washers and dispensers.

Note that 405 TS, 405 LS, EL406 and MultiFlo FX must be under computer control via LHC Secure software (sold separately) in order to work with barcode labeled plates and BioStack's barcode scanner.
EVENT PREVIEWS
BioTek Cytation Product Workshop and User Group Meeting
Experimental Biology 2015 | Sunday, March 29, 2015, 11:00 am - 1:00 pm

Are you attending Experimental Biology? Come hear about BioTek's breakthrough product, the Cytation 5 Cell Imaging Multi-Mode Reader and the cutting edge science being performed by our customers from both academia and industry. Talks include:

Cytation 5Joseph Haegele, Cornell University
SCIENCE TAPAS: How one plate has so many uses!
High-throughput screening and quantitative biochemical assays have become the mainstays of cutting-edge academic research in Chemical Biology. We utilize the BioTek Cytation 3 for pioneering assays developed in house for evaluating (1) the anticancer nucleotide-driven process of inhibition-coupled protein oligomerization via Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Polarization (FP) (2) the upregulation of therapeutically beneficial transcriptional responses via bioluminescence, (3) the drug-induced changes in nucleocytoplasmic protein distribution via fluorescence microscopy, and (4) the status of cellular reducing capacity and thus viability via fluorescence.
Neil Emans, Persomics, Inc.
Miniaturized RNAi Screening Technology: Printed Libraries Imaged With Cytation 3
RNA interference is routinely used in High-content and Phenotypic screening. However, set-up and operational costs remain beyond the scope of individual labs. Persomics technology miniaturizes, accelerates and deindustrializes RNAi screening enabling anyone to screen with an automated microscope. This lowers the cost and time for any scale of screening, enabling individual labs or facilities to do more. This presentation will introduce the Persomics platform together with current and future applications.
Scott Sneddon, SharpEdge Labs
New Protein Trafficking Assays by Image Cytometry
Protein trafficking assays, especially the tracking of cell surface receptors, ion channels and transporters to and from the plasma membrane, have typically required either quantitative western blot analysis or high content imaging followed by colocalization analysis. The recently introduced Fluorogen Activating Peptide tagging technology allows cellular trafficking assays to be performed using homogeneous readouts, without the need for complex cellular analysis. We will present data demonstrating cellular trafficking assays for CFTR (the chloride channel with defective trafficking in Cystic Fibrosis) as well as in hERG (important in Cardiotox) and PgP (important in cancer drug resistance).
Brad Larson, BioTek Instruments
Correlation of Kinetic Inhibitory Ligand Binding with 3D Tumor Invasion for the Evaluation of anti-Metastatic Effects of CXCR4 Inhibitors
Here we examine the drug-target residence time of various CXCR4 inhibitors using a direct, homogeneous ligand binding assay and CXCR4 expressing cell line. This inhibitor panel was further tested in a 3D tumor invasion assay to determine whether there is a correlation between the molecule's CXCR4 residence time and inhibition of the phenotypic effect of tumor invasion.
Complimentary lunch will be served. Free gift to the first 50 attendees as well as a chance to win a set of Beats headphones.


Phenotypic and Mechanism of Action Analysis of Anti-Metastatic Molecules using 3D Spheroid-Based Tumor Invasion Assays, Kinetic Microplate Detection, and Cellular Microscopy

AACR 2015 | Tuesday, April 21, 2015, 3:00 pm - 4:00 pm
Speaker: Brad Larson, Principal Scientist, BioTek Instruments, Inc.
In this presentation we will demonstrate a method for the generation of 3D spheroidal tumoroid structures, creation of a suitable invasion matrix, image-based monitoring of tumor invasion, and cellular analysis of captured images. Spheroid Microplates, coated with an ultra low attachment surface, were incorporated for tumoroid formation, and performance of the invasion process. Tumor invasion tracking was performed via digital microscopy using a novel cell imaging multi-mode reader. We will also demonstrate the ability to examine drug-target residence time of inhibitors using a direct, homogeneous ligand binding assay prior to phenotypic invasion analysis, in addition to analysis of the involvement of matrix metalloproteinases during the invasion process. The combination presents an accurate, yet easy-to-use method to assess target-based and phenotypic effects of new, potential anti-metastatic drugs.
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