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Customer Spotlights | Featured Application | Tek Corner | Product Spotlights 
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CUSTOMER SPOTLIGHTS

Identification and Characterization of Cancer Risk Factors Using High Throughput Cell Screening
The McManus Lab
Related ProductsBioSpa 8Cytation 3EL406


Dr Kirk McManus, Associate Professor in the Department of Biochemistry & Medical Genetics at the University of Manitoba and a Senior Scientist within the Research Institute in Oncology at CancerCare Manitoba, spoke to SelectScience® about his cutting-edge cancer research, which aims to identify cancer causing genetic abnormalities and screen potential therapies.

Dr McManus’ research is focused on two main areas:

  • The identification of aberrant genes that can predispose to cancer development or contribute to its progression
  • Screening putative cancer therapies that can exploit these genetic defects
The team use a variety of cell based assays and imaging methodologies to identify and investigate genetic risk factors that cause genome instability and predispose carriers to the development of cancer, as well as testing potential modifiers for therapeutic potential.

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Bringing Lab Quality Biodetection Technology to the Field
Black Ivory Biotech
Related Product: MultiFlo FX


SelectScience® spoke to Dr Gabriel Peckham, Principal Investigator, Black Ivory Biotech about his research into developing new transportable technologies for biodetection.

Black Ivory Biotech is focused on the development of “assays, software and electronic equipment for multiplex, low weight and power portable biodetection platforms”, which are “designed to be used by military, first responders and point-of-care technicians to detect high-consequence pathogens in people or the environment”, stated Dr Peckham.

Dr Peckham, whose previous work focused on anthrax detection, explained that the platforms are made to enable “high accuracy and sensitivity, but with a short sample-to-signal time,” an important consideration for the applications of this technology. “In these high-stress situations, it is important to have equipment that can be easily transported to the site of a potential threat without excessive weight and equipment and provide quality information quickly with minimal risk to the operator.”

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FEATURED APPLICATION

 

Oridonin Perfusion Causes Cytotoxicity in U-2 OS Cells
Using the ONIX2 System in Conjunction with the Lionheart™ FX Imager
 

The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples. Unfortunately, much of the work has been performed under static fluidic conditions that allow compound degradation products and cellular metabolites to build up. Here we describe the use of the Lionheart™ FX Automated Live Cell Imager and the CellASIC ONIX2 perfusion micro-incubator to rapidly image and analyze perfused tissue culture cells in multiple fluorescent colors and brightfield.

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TEK CORNER

 

Comparison of Oridonin Cytotoxicity in U-2 OS and HepG2 Cells
Using the BioSpa™ 8 to Manage Repeated Reagent Additions to Live Cells
 

The timed exposure of live cells to compound is a long standing experimental protocol for drug discovery. The speed and concentration at which compounds elicit a response to cells in vitro provide important clues as to their efficacy in vivo. Here we describe the use of a BioSpa™ 8 Automated Incubator to interface a MultiFlo™ FX Multi-Mode Dispenser to a Cytation™ 5 Cell Imaging Multi-Mode Reader to perform automated cytotoxicity testing.

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PRODUCT SPOTLIGHTS

Automate Live Cell Assays with the new BioSpa 8

Lionheart FXBioSpa 8 Automated Incubator gives your live cells the ultimate treatment... from the cozy, heated and humidified environment with CO2 and O2 control and monitoring, your live cells won’t need to lift a…microtubule(!)... to grow and thrive. Cells can relax, grow and be pampered – whether they’re in microplates, dishes or flasks. At feeding time, BioSpa 8 automatically moves the cells (in their vessels) to the MultiFlo FX Multi-Mode Dispenser or the EL406 Washer Dispenser - the cells aren’t stressed by unnecessary handling. Back into the BioSpa 8 drawer for more incubation time, then the plates are automatically moved to Cytation Cell Imaging Multi-Mode Reader…just in time to count them, capture brilliant images and analyze them with powerful Gen5 Software. BioSpa 8 manages all aspects of long or short term live cell imaging, continuously monitoring and controlling the environment to keep cells happy and healthy. It doesn’t get any better for your live cell assays! 

Gen5 3.0 Microplate Reader and Imager Software

Gen5New Gen5 3.0 offers a long list of great new features for live and fixed cell imaging and analysis with BioTek’s Lionheart FX and Cytation Imagers. Gen5 3.0 is offered in several editions to accommodate workflows and analysis from basic cell counting to the highly advanced image analysis offered in Gen5 Image Prime, including the ability to define a primary nuclear mask and a secondary mask for cytoplasm or whole cell measurements. With 3.0, you’ll be able to:

  • Capture kinetic images, automatically align them and generate a movie - no need to export to an external program.
  • Annotate images on-screen, saving them with the image - no need to export to an external program.
  • Make translocation measurements with custom object-level measurements - no need to export to an external program.
  • And so much more.
TEK TIPS

 Turn On/Turn Off the Vacuum Pump to drain the priming trough in EL406 and 405 LS
At the Quick menu:

  • Press Shift+1 to turn on the vacuum pump
  • Press Shift+2 to turn off the vacuum pump
  • Use these key sequences to manually control the vacuum pump when dispensing fluid. At times the priming trough fills up and is not emptied automatically. This option gives you control in that situation.

 Collect data from different plate types and save as a single Gen5 Experiment file in Gen5
A plate type with complex geometry requiring several plate definitions may need to be imaged, or it may be more convenient to store data from all 3 slides on a Take3 Trio in one Gen5 Experiment file.  This can be accomplished by setting up a Calibration Protocol.  Up to 10 Calibrator plates can be defined.  Each Calibrator plate can have a separate Procedure and Plate Type associated with it.

 Capture fast biological events in Gen5 with the new Burst mode
To image fast kinetic events, a high frame rate is required in order to capture the movement of signal. Gen5 3.0’s new burst mode feature allows you to capture full resolution images at speeds up to 10 frames per second. For an added burst of speed up to 20 frames per second, try turning on 2 x 2 pixel binning. Binning utilizes the CCD camera’s versatility to enhance readout speeds and improve signal to noise ratios.

FEATURED VIDEOS

Lionheart FX Automated Live Cell Imager
Debuts at Experimental Biology 2016

  Lionheart FX Automated Live Cell Imager Debuts at Experimental Biology 2016
Lionheart™ FX Automated Live Cell Imager enables superior digital microscopy with high resolution images up to 100x.

Live Cell Assay Automation from BioTek
LIVE at SLAS 2016

Live cell Assay Automation
Optimize live cell assay automation with BioTek’s Cytation 5 Cell Imaging Multi-Mode Reader and BioSpa 8 Automated Incubator.
NEW APPLICATION NOTES


 

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