TekTalk - Life Science Instrumentation News
Ptk2 cells (rat kidney) at 20x, image taken on Cytation 3 Cell Imaging Multi-Mode Reader. Cells fixed and stained for tubulin (green), actin (red) and nucleic (blue).
CUSTOMER SPOTLIGHTS

Time to Move Forward with Confidence in Results
Dr. Jorge Farías Avendaño, Director of Chemical Engineering Department, Faculty of Engineering and Sciences, Universidad de La Frontera, Temuco, Chile
Related ProductSynergy™ HTX


 Dr. Johnson with Miranda NeubertDr. Jorge Farías studies cellular and molecular mechanisms in fish spermatozoa as part of his role as the Director of Chemical Engineering at the Universidad de La Frontera in Temuco, Chile. Spermatogenesis, which is the development of sperm, is a delicate balance between cell proliferation, differentiation and apoptosis, and disruptions can have profound implications. For example, sperm cryopreservation is a basic tool in the field of animal improvement, however, cryodamage sustained during freezing and thawing can alter or disrupt cell structure and physiology, and impact both fertilization capabilities and the resulting quality of spawn. Internal factors, such as hypoxia, may also induce adverse effects. Dr. Farías’s research aims to create actionable insights toward developing potential targets for therapeutic strategies and modifying breeding practices to ensure the quality of offspring. Additionally, his lab investigates protein engineering in yeast such as P. pastoris to improve efficiency and cost-effectiveness in medical and pharmaceutical applications. This methylotrophic yeast is amenable to genetic manipulations, with rapid growth rates in high-density fermentations.

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Seeing a Clear Slice of Life in Crohn’s Disease
Dr. Gwendalyn Randolph
Related Product: Cytation 5

Dr. Gwen RandolphDr. Gwendalyn Randolph started her career studying immune cell trafficking in and out of tissues, particularly myeloid cells that initiate immune responses and those that derive from monocytes in the blood. In the chronic cardiovascular disease atherosclerosis, she wondered if there was an association between disease progression and the accumulation of myeloid cells because they couldn’t traffic out of the area. As part of this research, she looked at lymphatic vessels, as they drain fluid and traffic certain immune cells to lymph nodes.

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UPCOMING WEBINAR

Combining Automated Liquid Handling, Cellular Microscopy and Magnetic Bioprinting to Create Walk Away 3D Oncology, Stem Cell Differentiation, and Toxicity Procedures

Wednesday, May 24, 2017
12:00 - 1:00 PM EST


Presenters: Jan Seldin, Greiner Bio-One, Monroe, NC; Glauco Souza, n3D Biosciences, Inc., Houston, TX; Brad Larson, BioTek Instruments, Inc., Winooski, VTCell Counts

The use of cells cultured into a three-dimensional (3D) configuration continues to gain acceptance within multiple areas of life science research and drug discovery. Assay procedures commonly performed with two-dimensional (2D) cultured cells are rapidly migrating to 3D because of the reestablishment of cell-cell and cell-extracellular matrix (ECM) interactions, and in vivo-like results these cell models afford. Examples include cell migration studies, stem cell differentiation, and toxicity assessments. Magnetic bioprinting is a versatile method for aggregating cells and fomenting ECM interactions that can be used across multiple assay procedures. Using this technology, cells can be printed into 3D ring, dot, or spheroid structures after biocompatible magnetic particles are attached. In this webinar we will demonstrate how assay procedures using 3D magnetic bioprinting can be performed in a walk-away manner using automated liquid handling and incubation, and monitored through fluorescent and label-free image-based analysis. 
 

FEATURED APPLICATION

 

Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay

An in vitro model for Natural Killer (NK) cell mediated cytotoxicity was developed using a collagen-based scaffold 3D cell culture technology. This technology induced HCT116 cells to aggregate into tumoroids over time which became the target cells during the cytotoxicity assay. Cytotoxicity was assessed by both phosphatidyl serine exposure (apoptosis) and plasma membrane rupture (necrosis) using fully automated workflows. Cytotoxicity was quantified using NK cells alone and with IL-2 stimulation, where a significant increase of cytotoxicity was evident. Cytotoxicity with this model was compared to HCT116 cells adhered to microplates in a conventional 2D format. The 3D model was far superior in both maintaining cell health over time and accurately depicting cytotoxic events.
 
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TEK CORNER

 

Characterizing Calcium Mobilization using Kinetic Live Cell Imaging


Ca2+ acts as an important second messenger in diverse signaling pathways, including G protein-coupled receptors. Characterizing these pathways requires the ability to detect rapid changes in intracellular Ca2+ levels with high temporal resolution. Here we describe a live cell imaging based approach to quantify Ca2+ flux kinetics using the Lionheart™ FX and Fluo-4 Ca2+ indicator dye that delivers sub-second resolution and a large assay window.

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PRODUCT SPOTLIGHT

BioStack™ Microplate Stacker
Lionheart FX

BioTek’s Instruments help automate many routine laboratory processes, such as microplate washing and dispensing, multi-mode detection and cellular imaging. When the volume of work increases, you can achieve greater automation and higher throughput by integrating a BioStack Microplate Stacker to the system. BioStack is available in several models to cover the most common throughput needs, including important considerations like lidded microplate handing, super-fast processing speeds and compact size to fit in a biosafety enclosure. BioStack is easily integrated and software controlled for rapid implementation with any compatible BioTek instrument.


 
TEK TIPS

 Improve image quality by adjusting objective correction collars

BioTek imagers use several long working-distance objectives that are equipped with correction collars. These collars compensate for aberrations caused by differing bottom thicknesses of vessels. Before starting your imaging experiment, double check that the setting of your objective’s correction collar matches the bottom thickness of your imaging vessel. This will improve overall image quality and enhance image-based autofocus accuracy. For more information on properly setting the correction collar, please see the operator’s manual for your BioTek imager. (Operators manuals can be downloaded from the Customer Resource Center.)

 Lidded plate handling with BioStack 4
It’s possible to work with lidded and non-lidded microplates with BioStack 4. When integrated with BioTek’s imaging or detection systems, BioStack 4 processes are controlled through Gen5 software. MultiFlo FX and 405 TS can control BioStack 4 directly through their touchscreen interface, or via LHC Software.

 Prime time!

Keep your BioTek washer or dispenser performing well, even if it sits for several hours without use, by using the AutoPrime function. AutoPrime  periodically primes the fluidic path at a selectable interval. Consult your instrument’s Operator’s Manual for complete instructions for customizing and running the AutoPrime routine. (Operators manuals can be downloaded from the Customer Resource Center.)
FEATURED VIDEOS

BioStack Automation

BioStack
BioStack™ is a compact and versatile microplate stacker compatible with BioTek's washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Lionheart FX Automated Microscope

  Lionheart FX Automated Live Cell Imager
Traditionally, cellular processes have been visualized by fixation and staining of cells. However, live cell assays offer kinetic visualization and multi-parametric information concerning many biological processes. Lionheart FX Is an affordable, easy-to-use solution that also brings remarkable clarity to live cell microscopy.
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