TekTalk - Life Science Instrumentation News  
Ptk2 cells (rat kidney) at 20x, image taken on Cytation 3 Cell Imaging Multi-Mode Reader. Cells fixed and stained for tubulin (green), actin (red) and nucleic (blue).

Dreaming Big When It Comes to the Possibilities
Dr. Michael Johnson, Department of Immunobiology, University of Arizona
Related ProductCytation 5

 Dr. Johnson with Miranda NeubertMetals play a very important role in biology, as metal ions are associated with an estimated 40% of all proteins. In bacteria, dedicated acquisition and efflux systems tightly control metal homeostasis. Some metals, such as iron, are so critical to bacterial physiology that in most cases only the acquisition system exists; while others, such as copper, are so toxic that only efflux systems exist. It is this copper toxicity that is of particular interest to Dr. Michael Johnson and his new laboratory in the Department of Immunobiology at the University of Arizona’s College of Medicine. Dr. Johnson notes that although bacteria can develop resistance, as seen against modern antibiotic therapies, they remain vulnerable to the toxic effects of copper. His research aims to unravel this age-old mystery by understanding how copper is toxic to bacteria, and how bacteria overcome copper toxicity.

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Striking a Lethal Blow to Chemoresistance
Dr. Steve Quiros, University of Costa Rica
Related Product: Cytation 3

Dr. Steve Quiros
Malignant tumors are heterogeneous, demonstrating a diverse population of cell morphologies and phenotypic profiles. This results in specific cancer types expressing dissimilar characteristics from one patient to the next, and chemotherapies targeted towards a specific tumor may not eradicate the entire cellular mass. Dr. Steve Quiros and his colleague, Dr. Rodrigo Mora, at the Tumor Chemosensitivity Laboratory (LQT), Faculty of Microbiology at the University of Costa Rica are taking a close look at mechanisms to circumvent these hurdles and increase the effectiveness of chemotherapies, cancer diagnostic tools and personalized treatments. Specifically, Dr. Quiros focuses on temporal changes within the DNA damage response when exposed to genotoxins. He notes, “When DNA is damaged, an intracellular network is activated that coordinates a variety of responses within the cell, like repair pathways, arrest of the cell cycle and some metabolic changes that determine cell death or survival; and ultimately, we want to modulate this response to make malignant cells die.”

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"Kinetic Proliferation Assay Using High Contrast Brightfield Imaging"
Presenter: Joe Clayton, Ph.D., Principal Scientist, BioTek Instruments
Cell Counts

Characterizing cell proliferation is a crucial aspect of biological research and therapeutic drug development. Most current cell proliferation assays rely on indirect biochemical metrics that are limited by artifacts or imaging-based endpoint measures. In this webinar, we will describe a continuous live-cell assay for determining cell proliferation profiles using high contrast brightfield imaging. This fully automated method enables long-term quantitative analysis of cell growth using non-invasive methods to directly count cells or assess % confluence.

Wednesday, March 15, 2017
12:00 - 1:00 PM EST



Kinetic Proliferation Assay Using Label-Free Cell Counting

Kinetic cell proliferation assays enable quantitative analysis of cell viability and growth rates and are a powerful tool for evaluating cellular response to drug treatment. Conventional methods rely on either indirect biochemical measurements or the use of fluorescent labels. Alternatively, high contrast brightfield cell counting provides a direct and label-free method for measuring cell population size. Combining the high contrast brightfield imaging capabilities of the Cytation™ 5 Cell Imaging Multi-Mode Reader with the BioSpa™ 8 Automated Incubator enables convenient and robust long-term kinetic cell proliferation studies.
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A Combined, Affordable Solution for the Performance of Automated 3D in vitro Hepatotoxicity Testing

Prescription medications, environmental toxins, and non-prescription herbal remedies together form the major causes of hepatic injury. When looking at drugs alone, induced hepatic injury is the most common reason cited for warnings or withdrawal of an approved drug, depending on the severity of the induced hepatotoxicity. Here we demonstrate the ability to combine liquid handling with a novel cell imaging multi-mode reader to perform 3D hepatotoxicity studies.

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“Simply a good, fast, flexible and multi-use plate reader.” 
Lionheart FX

This is what one user said about Synergy Neo2 Multi-Mode Reader, launched in 2015. 

Synergy Neo2 was designed to provide the best combination of multi-mode detection performance, high throughput and high value for life science researchers.  The Synergy Neo2 has delivered on its promise of performance, speed and value, as noted by many favorable reviews  and awards including


We’re proud of the impact that Synergy Neo2 has had on our customers’ research exemplified by these two awards voted on by scientist like yourself. With applications that span nucleic acid quantification to ADME/Tox to HTS screening. Synergy Neo2 can make quick work of many assays in a single plate, or in a stack of plates fed quickly and efficiently by its integrated stacker for up to 50 plates at a time. Synergy Neo2 is also compatible with the BioSpa Automated Incubator for live cell assay workflows. Learn more about how Synergy Neo2 can help facilitate you  multi-mode detection workflows.

Patented Cytation Cell Imaging Multi-Mode Reader

BioTek was recently awarded a patent for Cytation Cell Imaging Multi-Mode Reader. Cytation’s unique design incorporates digital widefield microscopy and multi-mode detection in a single platform. 

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 Easily share your image data with collaborators

Gen5Gen5 makes it easy to share large image datasets with built-in file compression. This feature allows you to save an experiment and all associated images as a single, convenient Zip file. To use, save your current Gen5 session and then select “Create Zip File…” from the File menu at the top of the screen. Next, choose the images you wish to include in the Zip file. If you don’t need to share all of your images, save some space by selecting the image set, plate and/or wells you wish to include before clicking OK. You can also choose to include transformed images sets generated from image processing steps such as stitching or the raw images only. Finally, choose a location on your computer to save the Zip file and let Gen5 take care of the rest.

 Gen5 allows you to add a Take3 or Take3 Trio plate without connecting to an instrument by simulating the alignment process 

From the top menu bar in the main Gen5 screen go Take3  Take3 Plates... → enter a number in the “Serial Number” field → click “Align with Reader...” → press Ctrl + Shift + x → Click on the “Sim” button that should appear in the window.  You will now be able to simulate a measurement in the Gen5 Take3 interface - a great way to become familiar with how this part of Gen5 works.

 Maximize the performance of your cassette

There are several things you can do to maximize the performance of your dispense cassette.  Filtering supply fluids to 50 microns will help prevent clogs.  Purge and Prime the cassette if the dispenser has been idle for more than 20 minutes to eliminate bubbles.  Always allow the cassette to cool to room temperature after autoclaving. Finally, when finished using the cassette, always Purge the lines and then backflush each tube. Backflush from the dispense tip using the provided 10 cc syringe.  First push deionized water through each line, then push through air to displace the water.  These few things will help keep your cassette in top condition. 

Live Cell Imaging and Analysis with Lionheart FX

  Lionheart FX Automated Live Cell Imager
Learn how the Lionheart FX Automated Live Cell Imager’s integrated design and environmental controls make it ideally suited for short and long-term kinetic live cell applications.

Lionheart FX and Gen5 Software:
15 Minute Setup

Live cell Assay Automation
See how easy it is to get setup with the Lionheart FX Automated Live Cell Imager and Gen5 Software.


High Magnification Oil Immersion Imaging in Lionheart FX

  Lionheart FX Automated Live Cell Imager
Learn more about air and oil immersion imaging with BioTek’s Lionheart FX Automated Live Cell Imager and Gen5 Software.

Think Possible

  Lionheart FX Automated Live Cell Imager
When you think of the future, what do you think is possible? At BioTek we're constantly innovating to provide Scientists with state of the art instrumentation to help them in the discovery process...

Scientifically Speaking

  Lionheart FX Automated Live Cell Imager Debuts at Experimental Biology 2016
Live cell Assay Automation

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